Appnr

Graphical design of primers for PCR

PerlPrimer is a free, open-source GUI application written in Perl that designs
primers for standard Polymerase Chain Reaction (PCR), bisulphite PCR,
real-time PCR (QPCR) and sequencing. It aims to automate and simplify the
process of primer design.

If operated online, the tool nicely communicates with the Ensembl
project for further insights into the gene structure, i.e., allowing
for taking the location of exons and introns into account for the design
of the primers. The sequences themselves can be retrieved, too.